Among the following double-stranded DNA fragments (each 6 base pairs, one strand shown 5^prime to 3^prime), which has the highest melting temperature? (Use hydrogen bonds: A-T = 2, G-C = 3.)

JEE Main levelSingle correct optionDifficulty 2 of 3bm151

Among the following double-stranded DNA fragments (each $6$ base pairs, one strand shown $5^{'}$ to $3^{'}$ ), which has the highest melting temperature? (Use hydrogen bonds: A-T = 2, G-C = 3.)

Higher GC content means more hydrogen bonds and higher

T_{m}

. GCGCGC has

6 \times 3 = 18

bonds, GGATCC and CCGGAT have 16 each and ATATAT has 12.