JEE Main levelSingle correct optionDifficulty 2 of 3bm149
Bacterial DNA labelled with 15N in both strands is allowed to replicate semi-conservatively for exactly two rounds in a medium containing only 14N. The four resulting DNA duplexes are
Aall four with one 15N strand and one 14N strand
Bone with both strands 15N and three with both strands 14N
Ctwo with one 15N strand and one 14N strand, and two with both strands 14N
Dall four with both strands 14N
Answer: C
After round 1 both duplexes are hybrids (15N/14N). In round 2 each hybrid separates: the heavy strand makes a hybrid and the light strand makes a fully light duplex. This gives 2 hybrids and 2 fully 14N duplexes.