Answer: A, C, D
A tripeptide has two peptide bonds, formed with loss of two molecules of water (not three). The residues are Ala, Ser and Gly; the C-terminal residue, glycine, is achiral. Alanine and serine each contribute one stereocentre, so there are two in total (RDKit count: 2). The tripeptide is Ala-Ser-Gly.